Doubled haploid (DH) technology provides important benefits to maize breeding programs, as DH lines enhance genetic gains and improve breeding efficiency in addition to offering significant economic advantages. DH lines can be generated in less than half as many seasons as lines generated through traditional breeding, thus saving valuable time, resources and energy. The large scale production of DH lines is dependent on the identification of haploids at an early seedling stage. Haploids have traditionally been identified by the R1-nj (Navajo) anthocyanin color marker which, when crossed with haploid inducer lines through induction crosses, will produce haploid progeny seed with purple markings on its outer layer, or pericarp. This is to differentiate haploid from diploid seedlings, which are not useful for DH breeding.
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